Bacteria: Coliform & E Coli in Water Test Kit (487197) Result 24 Hours

Model #: 487197
This item No Longer Available.  eMail sales at for additional information or replacement product advice.

Switch to Desktop View

Print Friendly View
Click Here to Contact Us for the replacement part number and pricing Bacteria: Coliform & E Coli in Water Test Kit (487197) Result 24 Hours
Click to Enlarge

First Product Previous Item Product 79 of 89 Next Item Last Product
Category Water Testing

Coliform bacteria produce the enzyme β-D-galactosidase which splits the enzyme substrate 5- bromo-4-chloro-3-indolyl-β-D-galactoside (X-GAL). A colored end product is produced; if coliforms are present the liquid sample turns a blue-green color.

E. coli produces the enzyme β3 -D-glucuronidase, which breaks down the enzyme substrate 4- methylumbelliferyl-β-D-g1ucuronide (MUG) to generate an end product. The sample is illuminated with a UV light at about 365nm; if E. coli is present the sample will glow a bright blue, fluorescent, color.


  • Wash your hands thoroughly with soap and water
  • Carefully remove the bottle cap; do not touch the inside of the cap or bottle opening. Fill with l00mls of the water sample to be tested (about ½" below the neck of the bottle). Do not overfill. Securely recap the bottle.
  • Shake the bottle vigorously until all the media is dissolved. The solution should be clear yellow in color (a bit less clear with turbid samples)
  • Incubate the sample for:
    • 24 hours at 95° F (35° C) or
    • 48 hours at 77° F (25° C)

Coliform Confirmation:

  • After the 24 hour or 48 hour incubation time (based on temperature) observe the color of the sample:
    • Clear yellow negative for coliforms
    • Blue-green = positive for coliforms

E. coil Confirmation:

  • Shine a UV light (approximately 365nm) from the bottom of the sample. (Avoid looking directly at the light).
    • No fluorescence negative for E. coli
    • Bright blue fluorescence = positive for E. coli .

If further confirmation of E. coli is desired add 1 ml of Kovacs Indole reagent (sold separately) to the sample, recap the bottle, and wait 30 seconds. A red ring will form if E. coli is present.

UPC: 809762871978
Search Category: Hardware > Tools > Measuring Tools & Sensors

Air Quality - IAQ

Anemometers-Air Flow

Carbon Monoxide (CO)

EMF / Microwave Meters

Fire and Smoke Safety

Food Safety & Hygiene

Gas and Leak Detectors

Heat Detector Testing

Heat Index Monitors

Hygrometers / Humidity

Inspection Supplies

Light, LUX, UV Meters

Manometers / Pressure

Measuring Instruments

Meter Accessories

Moisture Meters

Power & Circuit Testers

Radon Mitigation & Fans

Radon Testing & Kits

Refractometers - BRIX

Safety and Security

Smoke Trace Emitters

Soil & Grain Meters

Sound Level Meters

Specialty Instruments


Thermometers Infrared

Tools and Hardware

Water & Flood Alarms

Water Testing

Promotional Items

Outlet / Overstock
Quick Find

Search Catalog:

Advanced Search
eMail Address:
Forgot Password?
Password Reset
Customer Reviews Click for the BBB Business Review of this Mail Order & Catalog Shopping in Marshall NC
Customer Service Radon Results Lookup
About Us Carbon Monoxide Gas
Shipping Policy Conversion Calculators
Return Policy Copyright & Trademark
Privacy Notice SPECIALS
Conditions of Use Radon Gas Information
Contact Us CO Detector Badge

Your IP Address is:
Copyright Inspect USA®. All rights reserved. 1995-2020
No part of this website may be reproduced without our express consent.
Distributors of TestKits, Inspection Tools, HVAC Smoke & Food Safety Equipment.