Special Order Item. Full cases of 12 only.
Allow 14 to 21 days for delivery.
Coliform bacteria produce the enzyme β-D-galactosidase which splits the
enzyme substrate 5- bromo-4-chloro-3-indolyl-β-D-galactoside (X-GAL). A colored
end product is produced; if coliforms are present the liquid sample turns a
E. coli produces the enzyme β3 -D-glucuronidase, which breaks down the enzyme
substrate 4- methylumbelliferyl-β-D-g1ucuronide (MUG) to generate an end
product. The sample is illuminated with a UV light at about 365nm; if E. coli is
present the sample will glow a bright blue, fluorescent, color.
48 hours at
77° F (25° C)
E. coil Confirmation:
If further confirmation of E. coli is desired add 1 ml of Kovacs
Indole reagent (sold separately) to the sample, recap the bottle, and wait 30
seconds. A red ring will form if E. coli is present.