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Bacteria in Water Test Kit Coliform & E Coli (487197) 48 Hour Result

Model #: 487197
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First Product Previous Item Product 68 of 89 Next Item Last Product
Category Water Testing

Special Order Item. Full cases of 12 only.
Allow 14 to 21 days for delivery.

Coliform bacteria produce the enzyme β-D-galactosidase which splits the enzyme substrate 5- bromo-4-chloro-3-indolyl-β-D-galactoside (X-GAL). A colored end product is produced; if coliforms are present the liquid sample turns a blue-green color.

E. coli produces the enzyme β3 -D-glucuronidase, which breaks down the enzyme substrate 4- methylumbelliferyl-β-D-g1ucuronide (MUG) to generate an end product. The sample is illuminated with a UV light at about 365nm; if E. coli is present the sample will glow a bright blue, fluorescent, color.


  • Wash your hands thoroughly with soap and water
  • Carefully remove the bottle cap; do not touch the inside of the cap or bottle opening. Fill with l00mls of the water sample to be tested (about ½" below the neck of the bottle). Do not overfill. Securely recap the bottle.
  • Shake the bottle vigorously until all the media is dissolved. The solution should be clear yellow in color (a bit less clear with turbid samples)
  • Incubate the sample for:
    • 24 hours at 95° F (35° C) or
    • 48 hours at 77° F (25° C)

Coliform Confirmation:

  • After the 24 hour or 48 hour incubation time (based on temperature) observe the color of the sample:
    • Clear yellow negative for coliforms
    • Blue-green = positive for coliforms

E. coil Confirmation:

  • Shine a UV light (approximately 365nm) from the bottom of the sample. (Avoid looking directly at the light).
    • No fluorescence negative for E. coli
    • Bright blue fluorescence = positive for E. coli .

If further confirmation of E. coli is desired add 1 ml of Kovacs Indole reagent (sold separately) to the sample, recap the bottle, and wait 30 seconds. A red ring will form if E. coli is present.

UPC: 809762871978
Search Category: Hardware > Tools > Measuring Tools & Sensors
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