3M™ Gluten Protein ELISA Kit, E96COC, 96 wells/kit
Ships out within 3 days. Perishable item. Only ships Next
Day Air. To prevent delay, select Next Day Air at checkout.
Since refrigeration is required, we ship swabs Monday through Thursday only (to prevent the parcel
from being left in terminal over the weekend). We include thermal wrap and/ or ice
pack when necessary.
- Designed to detect both processed and unprocessed target allergen
- Test can accommodate environmental swab, CIP rinse water and food
- A universal extraction protocol streamlines testing for multiple
analytes, saving you reagents and valuable time.
- 3M™ Environmental Collection Swabs sold separately.
The 3M™ Allergen Protein ELISA Kits are intended for the detection of
specific proteins in clean-in-place (CIP) final rinse water, environmental swab
samples, food ingredients and processed food products.
The 3M Allergen Protein ELISA Kits utilize a sandwich ELISA. The target
protein present in the sample react with the specific antibodies, which have
been adsorbed to the surface of polystyrene microtiter wells. After the removal
of unbound proteins by washing, antibodies conjugated with horseradish
peroxidase (HRP) are added. This enzyme-labeled antibody forms complexes with
the previously bound target protein. Following a second washing step, the enzyme
bound to the immunosorbent is detected by the addition of a chromogenic
substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The color development from this
enzymatic reaction varies directly with the concentration of target protein in
the sample tested; thus, the absorbance, at 450 nm, is a measure of the
concentration of the target protein in the test sample. The quantity of protein
in the test sample can be extrapolated from the standard curve, constructed from
standards of known concentration, and adjusted to consider the sample dilution.